Agar-gelatin for embedding tissues prior to paraffin processing

Agar-gelatin for embedding tissues prior to paraffin processing.

Benchmarks Fixed tissues often need to be arranged in a particular configuration , order, or orientation prior to processing for paraffin embedding. Although it is possible to arrange tissues after infiltration (during the final stages of paraffin embedding), it can be more convenient and practical to do this at the time of dissection or tissue collection. Agar has been the classical medium use...

Comparison of Paraffin Bait, Humic Acid Vitamin B Agar and Paraffin Agar Methods to Isolate Nocardia from Soil

Abstract Background and Objective: The Isolation of Nocardia species is complex and time-consuming, which is due to rapid growth of adjacent bacteria. Because of the importance of a specific medium with the ability of controlling intrusive microorganisms, this study aimed at comparing three laboratory methods to introduce the reliable isolation technique for Nocardia species. Material and Met...

Nicholson in formalin - fixed , paraffin - embedded tissues prior to analysis by Western blotScEnrichment of PrP

Diagnosis of prion disease is primarily through immunodetection of the infectious agent. Typically, 2 distinct procedures are recommended for a definitive diagnosis, with immunohistochemistry and Western blot providing the most information as to the specific isolate in question. In the past, these approaches required formalin-fixed, paraffinembedded tissue and fresh or frozen tissue, respective...

A Rapid Method of Paraffin Embedding

Paraffin embedding technique for specimens obtained by vitrectomy.

Histologic and immunohistochemical preparation of pathologic fluids containing cellular material is difficult, because the techniques are limited by the amount of cellular material. Similar problems limit the preparation of vitrectomized material. Cytopreparatory techniques, including millipore filter and celloidin bag cell block, have been introduced since 1980 for diagnostic vitrectomies. The...